Methods Used for PGD
The classical methods used in PGD are: polymerase chain reaction (PCR), Sanger sequencing, fluorescence in situ hybridization (FISH) and comparative genome hybridization (CGH), as well as more recently: next-generation sequencing (NGS) and karyomapping. Non-invasive PGD has so far only been performed experimentally.
For a detailed description of the different PGD techniques, see:
Steinke, V., & Rahner, N. (2009). Medizinisch-naturwissenschaftliche Aspekte der Präimplantationsdiagnostik. In Präimplantationsdiagnostik (Ethik in den Biowissenschaften – Sachstandsberichte des DRZE Bd. 10), 41–45. Karl Alber. https://www.drze.de/publikationen/sachstandsberichte/band-10
Sciorio, R., Tramontano, L., & Catt, J. (2020). Preimplantation genetic diagnosis (PGD) and genetic testing for aneuploidy (PGT-A): status and future challenges. In Gynecological Endocrinology. 36(1), 6-11. https://doi.org/10.1080/09513590.2019.1641194
Moffat, R., Raggi, A., Sartorius, G., Conrad, B., & Sterthaus, O. (2020). Die Präimplantationsdiagnostik in der Schweiz heute. In Schweizer Zeitschrift für Gynäkologie. 02, 12-15. https://www.rosenfluh.ch/gynaekologie-2020-02/die-praeimplantationsdiagnostik-in-der-schweiz-heute